Navigating the Challenges of Studying the Microbiome in Mouse Models: Design, Execution and Utility
Preclinical in vivo studies utilizing the mouse as a model organism are critical to advancing our knowledge of how the microbiome functions in disease, health and nutrition. These models will also be critical in supporting INDs and other regulatory requirements. Journal editors and reviewers are moving towards requiring authors to provide supporting information on how the microbiome affects research results.
This workshop will be moderated by Taconic’s Dr. Alex Maue, Director of Microbiome Products and Services. We are honored to announce that the workshop will be led by Dr. William DePaolo, Associate Professor of Medicine and the Lynn M. & Michael D. Garvey Endowed Chair in Gastroenterology at the University of Washington. Dr. DePaolo is also Director, Center for Microbiome Sciences & Therapeutics (CMiST). Assisting Dr. DePaolo will be a panel of scientists with diverse perspectives and includes Dr. James Matumba, co-founder Commense, Inc., Dr. Beth McCormick, professor and vice chair, University of Massachusetts Medical School, Founder Center for Microbiome Research and Dr. Phil Dube, Field Application Scientist, Taconic Biosciences. Join us as Dr. DePaolo leads us through a conversation on developing best practices for in vivo microbiome studies.
Studying the microbiome provides immense opportunity to improve human health. There is a growing realization that the microbiome provides a fertile opportunity for discovery of therapies from fecal transplants to bioactive molecules. Even as some microbiome based drugs move through clinical trials and regulatory approval, translatable, in vivo mouse studies are still in their infancy as regards standardization, best practices and reproducibility.
The experimental design variables in conducting in vivo studies are many; adding the complexity of the microbiome makes these studies even more challenging. Without established, predictive models, translational studies will be difficult at best and worse yet, inaccurate. Understanding best practices when planning these types of studies will be paramount in assuring reliable results that will support your drug development program. Hundreds of mouse microbiome studies have been published, wading through those to understand how best to set up your study is a daunting task.
This interactive workshop will provide the basis for planning and conducting successful microbiome studies in mice. Attendees will come away with the following understanding:
→ The considerations in selecting the appropriate in vivo model for your studies. Model choices include standard inbred or outbred, genetically engineered, germ-free, antibiotic-depleted or defined flora models or combinations. Understanding the utility of each will help you make the best choice for your specific study.
→ Microbiome association study best practices. Assure that donor microbiome remains viable and consistent during collection, storage and preparation. How best to collect and store samples during the in-life phase of the study.
→ The potential and limitations of data generated from mouse microbiome studies in the drug discovery. Learn how to identify risk and strategies to mitigate it.